JHSM
Journal of Health Sciences and Medicine

Journal of Health Sciences and Medicine (JHSM) is an unbiased, peer-reviewed, and open access international medical journal. The Journal publishes interesting clinical and experimental research conducted in all fields of medicine, interesting case reports, and clinical images, invited reviews, editorials, letters, comments, and related knowledge.

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Original Article
The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory
Abstract
Aims: To assess the viability of replacing serum samples with plasma samples in various clinical chemistry and immunoassay tests and to examine the implications of turnaround time (TAT) and sample quality during the transition process.
Methods: We compared the results of 27 paired clinical chemistry and 13 immunoassay tests from samples obtained using gel separator serum and gel separator lithium heparinized plasma (LIH) tubes. We used regression analysis, bias values, and Bland-Altman plots to compare the performance of serum and LIH tubes in various clinical chemistry and immunoassay tests. We collected and evaluated sample aspiration errors, hemolysis index values, and TAT data from the laboratory information system before and after switching to plasma in our study.
Results: Most tests showed no significant difference between the serum and LIH. However, for some analytes, total error (TE) values exceeded the total allowable error (TEa) limits derived from the biological variation database. Notably, insulin TE value did not exceed TEa, but it consumed near all its error budget. Consequently, we determined the alternative allowable error limits for some tests and found that plasma tubes could be used instead of serum tubes for most tests, except for lactate dehydrogenase (LDH). Plasma tubes improved the sample quality, reduced the incidence of aspiration errors, and decreased TAT in the emergency laboratory. We observed significant reductions in TAT after switching to plasma tubes.
Conclusion: Our study showed that LIH tubes can replace serum tubes in most clinical chemistry and immunoassay tests. Using LIH tubes in clinical laboratories can improve healthcare quality and reduce the workload of the laboratory staff.

Keywords : Plasma, serum, total error, turnaround time, sample quality

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Article available in :
Volume 6, Issue 5, 2023
Page : 998-1009
https://doi.org/10.32322/jhsm.1341282
Article Information
Received : 11 Ağu 2023
Accepted : 03 Eyl 2023
Published : 28 Eyl 2023
Corresponding Author :

Oğuzhan Zengi: Department of Medical Biochemistry, Başakşehir Çam and Sakura City Hospital, İstanbul, Turkey

[email protected]
Citation : Zengi O. The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory. J Health Sci Med. 2023;6(5):998-1009.

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