JHSM
Journal of Health Sciences and Medicine

Journal of Health Sciences and Medicine (JHSM) is an unbiased, peer-reviewed, and open access international medical journal. The Journal publishes interesting clinical and experimental research conducted in all fields of medicine, interesting case reports, and clinical images, invited reviews, editorials, letters, comments, and related knowledge.

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Original Article
Does ramelteon have an ameliorative effect in MTX-induced testicular injury?
Abstract
Aims: The aim of this study was to investigate the potential protective effect of Ramelteon (RMT), which exhibits antioxidant, anti-inflammatory and antiapoptotic properties, against testicular damage induced by Methotrexate (MTX), which is widely used in the treatment of various diseases, including chemotherapy.
Methods: 32 Wistar albino rats were equally divided into four groups: Control (group I), MTX (group II), MTX+RMT (group III) and RMT (group IV). Histologic evaluation was performed using Hematoxylin and Eosin (H&E) staining, immunohistochemical analysis using TNF-alpha and Cas-3, and biochemical evaluation using TAS, TOS and OSI.
Results: Histologic analysis using H&E staining revealed a significant difference between group I and groups II and III (p<0.05), while no significant difference was observed between group IV and the other groups (p>0.05). While normal histologic structures were observed in groups I and IV, histopathologic findings were noted in groups II and III. Immunohistochemical evaluation of TNF-alpha and Cas-3 showed a significant difference between group I and groups II and III (p<0.05), while no significant difference was observed between group IV and other groups (p>0.05). The highest immunostaining intensity was observed in group II. Biochemical evaluation revealed statistically significant differences in TAS, TOS and OSI parameters reflecting oxidative stress differences between the groups (p<0.05).
Conclusion: Anti-inflammatory, antioxidant and antiapoptotic properties of RMT demonstrated its protective effect against MTX-induced testicular injury in rats. Histological, immunohistochemical and biochemical analyses underline the potential role of RMT as a protective agent in MTX-induced testicular injury. This research aims to contribute to the understanding of potential applications to reduce the adverse effects of MTX therapy on reproductive health.

Keywords : Methotrexate, ramelteon, rat, testicular injury

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Article available in :
Volume 6, Issue 5, 2023
Page : 1034-1039
https://doi.org/10.32322/jhsm.1344540
Article Information
Received : 17 Ağu 2023
Accepted : 06 Eyl 2023
Published : 28 Eyl 2023
Corresponding Author :

Dilek Ulusoy Karatopuk: Department of Histology and Embryology, School of Medicine, Süleyman Demirel University, Isparta, Turkey

[email protected]
Citation : Ulusoy Karatopuk D, Atasoy N, Kumbul Doğuç D. Does ramelteon have an ameliorative effect in MTX-induced testicular injury?. J Health Sci Med. 2023;6(5):1034-1039.

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