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Journal of Health Sciences and Medicine

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Original Article
Incorporating Nigella sativa nanoemulsion into gelatin-guar gum films for enhanced healing of wound infections
Abstract
Aims: This study aims to investigate the impact of incorporating Nigella sativa essential oil nanoemulsion (NSNE) into gelatin (Ge) and guar gum (GG)-based films at various concentrations (0%, 2%, 4%, and 6%), to evaluate the antimicrobial properties of the resulting films against common bacterial strains associated with wound infections, as well as to assess their effects on the physical and chemical properties of the films, and to create a biomaterial with the potential to be utilized as wound dressing, possessing optimal properties capable of accelerating wound infection healing.
Methods: The nanoemulsion (NE) was obtained through ultrasonic irradiation. Polydispersity index (PDI), zeta potential, and particle size of NE were measured. For film preparation, gelatin (Ge) and guar gum (GG) were used, incorporating NSNE at concentrations of 0%, 2%, 4%, and 6%. Mechanical properties were evaluated using a universal testing machine, film thickness with a micrometer, and crystalline structure through X-ray diffraction (XRD) analysis. Scanning electron microscopy (SEM) was utilized for microstructure examination, and hydrophobicity was assessed by contact angle measurements. Antimicrobial activity was determined via the disk diffusion method against bacteria relevant to wound infections. Statistical analysis employed one-way ANOVA and Tukey post hoc tests with a significance level set at 5%.
Results: The particle size, PDI, and zeta potential of the NE were measured as 296±4.85 nm, 0.569±0.2, and -35.2±07 mV, respectively. The incorporation of NSNE into GE-GG-based films demonstrated promising antimicrobial efficacy against common wound infection bacteria, including Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, and Klebsiella pneumoniae. The films maintained mechanical integrity, with no significant alterations in tensile strength (TS) and elongation at break (EAB) (p0.05). However, higher NSNE concentrations led to decreased hydrophobicity (p<0.05) and structural changes, as evidenced by increased pores and cracks observed in SEM images.
Conclusion: This study highlights the potential of NSNE-loaded films to assist in healing wound infections, combining antimicrobial properties with a biocompatible film matrix.

Keywords : Biomedical engineering, wound infection, nanoemulsion, Nigella sativa

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Article available in :
Volume 7, Issue 2, 2024
Page : 146-152
https://doi.org/10.32322/jhsm.1419346
Article Information
Received : 13 Oca 2024
Accepted : 01 Şub 2024
Published : 25 Mar 2024
Corresponding Author :

Neslihan Mutlu: Department of Biology, Faculty of Sciences and Letters, Kafkas University, Kars, Turkiye

[email protected]
Citation : Mutlu N. Incorporating Nigella sativa nanoemulsion into gelatin-guar gum films for enhanced healing of wound infections. J Health Sci Med. 2024;7(2):146-152.

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