JHSM
Journal of Health Sciences and Medicine

Journal of Health Sciences and Medicine (JHSM) is an unbiased, peer-reviewed, and open access international medical journal. The Journal publishes interesting clinical and experimental research conducted in all fields of medicine, interesting case reports, and clinical images, invited reviews, editorials, letters, comments, and related knowledge.

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Original Article
Development and optimization of biotinoyl tripeptide-1 loaded solid lipid nanoparticles for protection against heat induced hair damage
Abstract
Aims: This study aimed to develop and optimize biotinoyl tripeptide-1 (BTP)-loaded solid lipid nanoparticles (SLNs) and to evaluate their protective efficacy against heat-induced structural and thermal damage in hair fibers.
Methods: A Box-Behnken experimental design was employed to optimize the formulation. The optimized BTP-SLNs were characterized for particle size, Polydispersity Index (PDI), zeta potential, and entrapment efficiency. Hair samples were exposed to repeated thermal cycles at 200°C over 15 days to simulate the thermal straightening damage. Morphological and thermal damage were also investigated using scanning electron microscopy (SEM) and differential scanning calorimetry (DSC).
Results: The optimized BTP-SLN formulation demonstrated a particle size of 207.3 nm, PDI of 0.297, zeta potential of -10.6 mV, and an entrapment efficiency of 46.2%. Heat-treated control samples exhibited pronounced cuticle lifting, cortical disruption, and decreased ?-keratin denaturation enthalpy. Empty-SLNs provided partial surface-level protection; the incorporation of BTP significantly enhanced thermal resistance. Additionally, BTP-SLN-treated hair showed improved cuticle integrity, reduced microfibrillary separation, increased denaturation peak intensity, and delayed onset of thermal degradation.
Conclusion: BTP-loaded SLNs demonstrated protective effects against heat-induced hair damage, likely through a dual mechanism involving lipid barrier formation and stabilization of the keratin matrix. These findings suggest that BTP-SLNs represent a promising strategy for improving thermal protection in cosmetic hair care applications.

Keywords : Biotinoyl tripeptide-1, solid lipid nanoparticles, hair, heat damage, DSC, SEM

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Article available in :
Volume 9, Issue 3, 2026
Page : 761-766
https://doi.org/10.32322/jhsm.1901401
Article Information
Received : 02 Mar 2026
Accepted : 08 May 2026
Published : 19 May 2026
Corresponding Author :

Mahmut Ozan Toksoy: Department of Pharmaceutical Technology, Faculty of Pharmacy, Dicle University, Diyarbakır, Turkiye

[email protected]
Citation : Toksoy MO, Şeker U, Akkuş Arslan Ş. Development and optimization of biotinoyl tripeptide-1 loaded solid lipid nanoparticles for protection against heat induced hair damage. J Health Sci Med. 2026;9(3):761-766. doi:10.32322/jhsm.1901401

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